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rapid barcoding kit  (Oxford Nanopore)
90
Oxford Nanopore rapid barcoding kit
Rapid Barcoding Kit, supplied by Oxford Nanopore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rapid barcoding kit/product/Oxford Nanopore
Average 90 stars, based on 1 article reviews
rapid barcoding kit - by Bioz Stars, 2026-06
90/100 stars
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oxford nanopore rapid barcoding kit  (Oxford Nanopore)
90
Oxford Nanopore oxford nanopore rapid barcoding kit
SARS-CoV-2 diagnosis and variant identification by the NIRVANA protocol. RNA samples were subjected to reverse transcription, followed by multiplex RPA to amplify five regions of the SARS-CoV-2 genome and the internal control ACTB. For sequencing, the amplicons were purified and prepared for nanopore sequencing using an optimized <t>barcoding</t> library preparation protocol. Sequencing was performed in the pocket-sized Nanopore MinION sequencer and sequencing results were analyzed by the algorithm termed RTNano on the fly. ( A ) Representative agarose gel electrophoresis result of multiplex RPA products from one positive sample. ( B ) Diagram of the genomic location of RPA amplicons and the number of SARS-CoV-2 DNMs (defining nucleotide mutations) covered by them. The IGV track shows sequencing coverage of the SARS-CoV-2 amplicons and the internal control ACTB amplicon in one sample. C. Read counts of the five SARS-CoV-2 amplicons and ACTB for the sequenced samples. D. Detected defining mutations and identified variants in the positive samples. POS. 1–12 are abbreviations of individual positive samples. The SARS-CoV-2 genome sequencing data of samples POS. 10, 11, and 12 have been deposited to the GISAID with IDs EPI_ISL_15827169 (POS. 10), EPI_ISL_15826832 (POS. 11), and EPI_ISL_15826930 (POS. 12). The original gels related to ( A ) are presented in Supplementary Material .
Oxford Nanopore Rapid Barcoding Kit, supplied by Oxford Nanopore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/oxford nanopore rapid barcoding kit/product/Oxford Nanopore
Average 90 stars, based on 1 article reviews
oxford nanopore rapid barcoding kit - by Bioz Stars, 2026-06
90/100 stars
  Buy from Supplier

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SARS-CoV-2 diagnosis and variant identification by the NIRVANA protocol. RNA samples were subjected to reverse transcription, followed by multiplex RPA to amplify five regions of the SARS-CoV-2 genome and the internal control ACTB. For sequencing, the amplicons were purified and prepared for nanopore sequencing using an optimized barcoding library preparation protocol. Sequencing was performed in the pocket-sized Nanopore MinION sequencer and sequencing results were analyzed by the algorithm termed RTNano on the fly. ( A ) Representative agarose gel electrophoresis result of multiplex RPA products from one positive sample. ( B ) Diagram of the genomic location of RPA amplicons and the number of SARS-CoV-2 DNMs (defining nucleotide mutations) covered by them. The IGV track shows sequencing coverage of the SARS-CoV-2 amplicons and the internal control ACTB amplicon in one sample. C. Read counts of the five SARS-CoV-2 amplicons and ACTB for the sequenced samples. D. Detected defining mutations and identified variants in the positive samples. POS. 1–12 are abbreviations of individual positive samples. The SARS-CoV-2 genome sequencing data of samples POS. 10, 11, and 12 have been deposited to the GISAID with IDs EPI_ISL_15827169 (POS. 10), EPI_ISL_15826832 (POS. 11), and EPI_ISL_15826930 (POS. 12). The original gels related to ( A ) are presented in Supplementary Material .

Journal: Scientific Reports

Article Title: An open-source, automated, and cost-effective platform for COVID-19 diagnosis and rapid portable genomic surveillance using nanopore sequencing

doi: 10.1038/s41598-023-47190-w

Figure Lengend Snippet: SARS-CoV-2 diagnosis and variant identification by the NIRVANA protocol. RNA samples were subjected to reverse transcription, followed by multiplex RPA to amplify five regions of the SARS-CoV-2 genome and the internal control ACTB. For sequencing, the amplicons were purified and prepared for nanopore sequencing using an optimized barcoding library preparation protocol. Sequencing was performed in the pocket-sized Nanopore MinION sequencer and sequencing results were analyzed by the algorithm termed RTNano on the fly. ( A ) Representative agarose gel electrophoresis result of multiplex RPA products from one positive sample. ( B ) Diagram of the genomic location of RPA amplicons and the number of SARS-CoV-2 DNMs (defining nucleotide mutations) covered by them. The IGV track shows sequencing coverage of the SARS-CoV-2 amplicons and the internal control ACTB amplicon in one sample. C. Read counts of the five SARS-CoV-2 amplicons and ACTB for the sequenced samples. D. Detected defining mutations and identified variants in the positive samples. POS. 1–12 are abbreviations of individual positive samples. The SARS-CoV-2 genome sequencing data of samples POS. 10, 11, and 12 have been deposited to the GISAID with IDs EPI_ISL_15827169 (POS. 10), EPI_ISL_15826832 (POS. 11), and EPI_ISL_15826930 (POS. 12). The original gels related to ( A ) are presented in Supplementary Material .

Article Snippet: Samples were prepared for sequencing using the SARS-CoV-2 genome sequencing protocol midnight with Oxford Nanopore Rapid barcoding kit ( https://www.protocols.io/view/sars-cov2-genome-sequencing-protocol-1200bp-amplic-rm7vz8q64vx1/v6 ).

Techniques: Biomarker Discovery, Variant Assay, Reverse Transcription, Multiplex Assay, Control, Sequencing, Purification, Nanopore Sequencing, Agarose Gel Electrophoresis, Amplification